Z-LEHD-aminoluciferin
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1021.99 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Molecular weight 1021.99 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
Z-LEHD-aminoluciferin is a luminogenic substrate that measures caspase-9 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence LEHD in this substrate is specific for protease cleavage by caspase-9. Following caspase-9 activation, the LEHD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-9 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Z-LEHD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 97.848 µL | 489.242 µL | 978.483 µL | 4.892 mL | 9.785 mL |
5 mM | 19.57 µL | 97.848 µL | 195.697 µL | 978.483 µL | 1.957 mL |
10 mM | 9.785 µL | 48.924 µL | 97.848 µL | 489.242 µL | 978.483 µL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
Z-LETD-aminoluciferin | 362 | 499 | 18000 |
Z-IETD-aminoluciferin | 362 | 499 | 18000 |
Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
Z-VEID-aminoluciferin | 362 | 499 | 18000 |
Z-ATAD-aminoluciferin | 362 | 499 | 18000 |