Name: Z-LEHD-aminoluciferin
Brand: AAT Bioquest
SKU: 13213
Price: 724 USD
Availability: InStock

Z-LEHD-aminoluciferin

Z-LEHD-aminoluciferin is a luminogenic substrate that measures caspase-9 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence LEHD in this substrate is specific for protease cleavage by caspase-9. Following caspase-9 activation, the LEHD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-9 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13213	1 mg	Price

References

View all 50 references: Citation Explorer

Caspase-9 suppresses metastatic behavior of MDA-MB-231 cells in an adaptive organoid model.

Authors:

Falahi, Farzaneh and Akbari-Birgani, Shiva and Mortazavi, Yousef and Johari, Behrooz

Journal:

Scientific reports (2024): 15116

Roles of Cyt-c/Caspase-9/Caspase-3/Bax/Bcl-2 pathway in Cd-induced testicular injury in rats and the protective effect of quercetin.

Authors:

Yu, Wenjing and Zhu, Huali and Huang, Ruxue and Yan, Bingzhao and Xu, Bing and Shi, Yaning and Mao, Junbing and Liu, Zongping and Wang, Jicang

Journal:

Toxicon : official journal of the International Society on Toxinology (2024): 107561

Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics.

Authors:

Říhová, Kamila and Lapčík, Petr and Veselá, Barbora and Knopfová, Lucia and Potěšil, David and Pokludová, Jana and Šmarda, Jan and Matalová, Eva and Bouchal, Pavel and Beneš, Petr

Journal:

Journal of proteome research (2024): 2999-3011

A Non-Apoptotic Pattern of Caspase-9/Caspase-3 Activation During Differentiation of Human Embryonic Stem Cells into Cardiomyocytes.

Authors:

Ghorbani, Negar and Shiri, Mahshad and Alian, Maedeh and Yaghubi, Roham and Shafaghi, Mojtaba and Hojjat, Hamidreza and Pahlavan, Sara and Davoodi, Jamshid

Journal:

Advanced biology (2024): e2400026

Chimeric Antigen Receptor T Cell with an Inducible Caspase-9 Suicide Gene Eradicates Uveal Melanoma Liver Metastases via B7-H3 Targeting.

Authors:

Ventin, Marco and Cattaneo, Giulia and Arya, Shahrzad and Jia, Jingyu and Gelmi, Maria C and Sun, Yi and Maggs, Luke and Ksander, Bruce R and Verdijk, Robert M and Boland, Genevieve M and Jenkins, Russell W and Haq, Rizwan and Jager, Martine J and Wang, Xinhui and Ryeom, Sandra and Ferrone, Cristina R

Journal:

Clinical cancer research : an official journal of the American Association for Cancer Research (2024): 3243-3258

Physical properties

Molecular weight	1021.99
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	362
Emission (nm)	499

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 15, 2025