Z-LEHD-ProRed™ 620

ProRed™-derived protease substrates are colorless and non-fluorescent. Cleavage of blocking protease-cleavable peptide residue by caspases generates the strongly red fluorescent ProRed™ that can be monitored fluorimetrically at ~620 nm with excitation of ~530 nm. ProRed™-derived caspase substrates are the most sensitive red indicators for the fluorimetric detection of various caspase activities. This LEHD-ProRed™ substrate is specific for detecting caspase 9.

Protocol

SDS

Catalog	Size	Price	Quantity
13435	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-LEHD-ProRed™ 620 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	53.599 µL	267.996 µL	535.992 µL	2.68 mL	5.36 mL
5 mM	10.72 µL	53.599 µL	107.198 µL	535.992 µL	1.072 mL
10 mM	5.36 µL	26.8 µL	53.599 µL	267.996 µL	535.992 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1865.7 g/mol	=	mL	x	mM	=	mmol

References

View all 101 references: Citation Explorer

Fisetin induces apoptosis in human cervical cancer HeLa cells through ERK1/2-mediated activation of caspase-8-/caspase-3-dependent pathway

Authors:

Ying TH, Yang SF, Tsai SJ, Hsieh SC, Huang YC, Bau DT, Hsieh YH.

Journal:

Arch Toxicol (2012): 263

Andrographolide induces apoptosis in B16F-10 melanoma cells by inhibiting NF-kappaB-mediated bcl-2 activation and modulating p53-induced caspase-3 gene expression

Authors:

Pratheeshkumar P, Sheeja K, Kuttan G.

Journal:

Immunopharmacol Immunotoxicol (2012): 143

ECRG4 is a negative regulator of caspase-8-mediated apoptosis in human T-leukemia cells

Authors:

Matsuzaki J, Torigoe T, Hirohashi Y, Kamiguchi K, Tamura Y, Tsukahara T, Kubo T, Takahashi A, Nakazawa E, Saka E, Yasuda K, Takahashi S, Sato N.

Journal:

Carcinogenesis (2012): 996

Proteasome inhibition can impair caspase-8 Activation upon sub-maximal Stimulation of apoptotic tumour necrosis factor-related apoptosis inducing ligand (TRAIL) signalling

Authors:

Laussmann MA, Passante E, Hellwig CT, Tomiczek B, Flanagan L, Prehn JH, Huber HJ, Rehm M.

Journal:

J Biol Chem. (2012)

Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis

Authors:

Imre G, Heering J, Takeda AN, Husmann M, Thiede B, Zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dotsch V, Rajalingam K.

Journal:

EMBO J. (2012)

Physical properties

Molecular weight	1865.70
Solvent	DMSO

Spectral properties

Excitation (nm)	532
Emission (nm)	619

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 17, 2026