Z-LETD-FMK

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	654.69
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Example protocol

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Z-LETD-FMK stock solution

Add appropriate amount of DMSO into Z-LETD-FMK to make 2-5 mM Z-LETD-FMK stock solution.
Note Store the unused Z-LETD-FMK stock solution at -20 °C in single use aliquots

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline and should be optimized according to your needs.

Treat your samples as desired to induce apoptosis.
Add Z-LETD-FMK along with the treatment of your choice.
Note We recommend using 10-100 μM of final concentration of Z-LETD-FMK to inhibit caspase-8.
Note Optimal time and concentration for incubation needs to be determined experimentally.
Stain samples as you desired.
Monitor the fluorescence intensity with your choice of instrumentation.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-LETD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	152.744 µL	763.72 µL	1.527 mL	7.637 mL	15.274 mL
5 mM	30.549 µL	152.744 µL	305.488 µL	1.527 mL	3.055 mL
10 mM	15.274 µL	76.372 µL	152.744 µL	763.72 µL	1.527 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Inhibition of caspase 8 Activities in Jurkat cells with caspase 8 inhibitor Z-LETD-FMK (Cat.# 13307). Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 5 hours. The Z-LETD-FMK was added and co-incubated with staurosporine. The caspase-8 activity was detected with Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit (Cat.# 22816). The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with Flexstation 3 fluorescence microplate reader (Molecular Devices).

Figure 2. Chemical structure for Z-LETD-FMK.

References

View all 8 references: Citation Explorer

Rapamycin, an mTOR inhibitor, induced apoptosis via independent mitochondrial and death receptor pathway in retinoblastoma Y79 cell.
Authors: Wang, Yan-Dong and Su, Yong-Jing and Li, Jian-Ying and Yao, Xiang-Chao and Liang, Guang-Jiang
Journal: International journal of clinical and experimental medicine (2015): 10723-30

A polysaccharide from Sanguisorbae radix induces caspase-dependent apoptosis in human leukemia HL-60 cells.
Authors: Wu, Zhigang and Sun, Honghui and Li, Jingzhong and Ma, Chijiao and Zhao, Siqiao and Guo, Zheng and Lin, Yao and Lin, Yaping and Liu, Li
Journal: International journal of biological macromolecules (2014): 615-20

Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.
Authors: Barreiro-Iglesias, Antón and Shifman, Michael I
Journal: Enzyme research (2012): 835731

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells.
Authors: Ji, C and Ren, F and Xu, M
Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (2010): 821-7

[Synergistic effects and mechanisms of combined tumor necrosis factor-related apoptosis-inducing ligand and chemotherapeutic drugs or radiotherapy in killing laryngeal squamous carcinoma cells in vitro].
Authors: Zhang, Ming and Zhou, Liang
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2009): 565-70

Emodin azide methyl anthraquinone derivative triggers mitochondrial-dependent cell apoptosis involving in caspase-8-mediated Bid cleavage.
Authors: Yan, Yanyan and Su, Xiaodong and Liang, Yongju and Zhang, Jianye and Shi, Chengjun and Lu, Yu and Gu, Lianquan and Fu, Liwu
Journal: Molecular cancer therapeutics (2008): 1688-97

Caspase-9 takes part in programmed cell death in developing mouse kidney.
Authors: Araki, Takashi and Hayashi, Matsuhiko and Nakanishi, Keiko and Morishima, Nobuhiro and Saruta, Takao
Journal: Nephron. Experimental nephrology (2003): e117-24

Induction of apoptosis in human leukemia cells by the CDK1 inhibitor CGP74514A.
Authors: Dai, Yun and Dent, Paul and Grant, Steven
Journal: Cell cycle (Georgetown, Tex.) : 143-52