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Z-LETD-FMK
Z-LETD-FMK is a cell permeable, irreversible caspase protease 8 inhibitor that binds to the catalytic site of caspase- 8, which plays an important role in the induction of apoptosis. The binding of Z-LETD-FMK to caspase-8 inhibits the activity of this protease, enabling it to inhibit apoptotic events associated with caspase-8 activation. For inhibition of apoptosis, Z-LETD-FMK should be added at the same time that apoptosis is induced. Fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible inhibitors with no added cytotoxic effects.
Inhibition of caspase 8 Activities in Jurkat cells with caspase 8 inhibitor Z-LETD-FMK (Cat.# 13307). Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 5 hours. The Z-LETD-FMK was added and co-incubated with staurosporine. The caspase-8 activity was detected with Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit (Cat.# 22816). The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with Flexstation 3 fluorescence microplate reader (Molecular Devices).
Inhibition of caspase 8 Activities in Jurkat cells with caspase 8 inhibitor Z-LETD-FMK (Cat.# 13307). Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 5 hours. The Z-LETD-FMK was added and co-incubated with staurosporine. The caspase-8 activity was detected with Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit (Cat.# 22816). The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with Flexstation 3 fluorescence microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
133071 mg
Price
 
Physical properties
Molecular weight654.69
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 22, 2025