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zFluor™ 635 succinimidyl ester

Our zFluor™ serial dyes are developed to have the possibly highest photostability at a given wavelength compared to the other similar wavelength dyes on the market. zFluor™ 635 has similar spectral properties to the popular Cy5® (GE Healthcare) and Alexa Fluor® 647 (ThermoFisher). Its high thermal and photostability makes it an excellent choice for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM and STED. Under the same test conditions, zFluor™ 635 has much higher ozone stability than Cy5® and Alexa Fluor® 647, making it a much better choice for microarray and other biochip-based applications. This feature is extremely useful for fluorescence in-situ hybridization (FISH). In addition, zFluor™-labeled oligonucleotides and peptides are much brighter and more photostable than the ones labeled by Alexa Fluor® 647 and Cy5®. Similarly, zFluor™ 635-labeled oligos demonstrated higher signals than the corresponding conjugates prepared with either Cy5® or Alexa Fluor® 647 in our qPCR tests. The absorption and fluorescence of our zFluor™ 635 dyes are independent of pH in the range of pH 2 to 11. They are well excited at 633 nm of He-Ne laser, the 647 nm line of the Krypton-Ion laser or a diode-laser.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
zFluor™ 647 succinimidyl ester648668
zFluor™ 633 succinimidyl ester623639

Citations

View all 1 citations: Citation Explorer
Inner hair cell stereocilia are embedded in the tectorial membrane
Authors: Hakizimana, Pierre and Fridberger, Anders
Journal: Nature Communications (2021): 1--13
Page updated on November 12, 2024

Ordering information

Price
Unit size
Catalog Number1501
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1336.89

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.12

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

110,0001

Quantum yield

0.221

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501