zFluor™ 635 succinimidyl ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1336.89
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.12
Correction Factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	110,000¹
Quantum yield	0.22¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1336.89

Correction Factor (260 nm)

0.12

Correction Factor (280 nm)

0.09

Extinction coefficient (cm ^-1 M ^-1)

110,000¹

Quantum yield

0.22¹

Our zFluor™ serial dyes are developed to have the possibly highest photostability at a given wavelength compared to the other similar wavelength dyes on the market. zFluor™ 635 has similar spectral properties to the popular Cy5® (GE Healthcare) and Alexa Fluor® 647 (ThermoFisher). Its high thermal and photostability makes it an excellent choice for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM and STED. Under the same test conditions, zFluor™ 635 has much higher ozone stability than Cy5® and Alexa Fluor® 647, making it a much better choice for microarray and other biochip-based applications. This feature is extremely useful for fluorescence in-situ hybridization (FISH). In addition, zFluor™-labeled oligonucleotides and peptides are much brighter and more photostable than the ones labeled by Alexa Fluor® 647 and Cy5®. Similarly, zFluor™ 635-labeled oligos demonstrated higher signals than the corresponding conjugates prepared with either Cy5® or Alexa Fluor® 647 in our qPCR tests. The absorption and fluorescence of our zFluor™ 635 dyes are independent of pH in the range of pH 2 to 11. They are well excited at 633 nm of He-Ne laser, the 647 nm line of the Krypton-Ion laser or a diode-laser.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of zFluor™ 635 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	74.8 µL	374.002 µL	748.005 µL	3.74 mL	7.48 mL
5 mM	14.96 µL	74.8 µL	149.601 µL	748.005 µL	1.496 mL
10 mM	7.48 µL	37.4 µL	74.8 µL	374.002 µL	748.005 µL