Name: Zinquin AM *UltraPure grade*
Brand: AAT Bioquest
SKU: 21261
Price: 232 USD
Availability: InStock

Zinquin AM

UltraPure grade

Zinquin AM ester is a lipophilic, zinc-sensitive, cell-permeable fluorescent probe. It is retained in living cells because the AM ester is cleaved by cytosolic esterase to give Zinquin that carry a negative charge, preventing its efflux across the plasma membrane. Zinquin fluorescent probes may be loaded into cells by in the culture medium containing 5-40 μM Zinquin ethyl ester in PBS with calcium and magnesium (or in culture medium). Cells are normally incubated with the Zinquin ethyl ester for 15-30 minutes at 37 °C. Exact loading concentration, time and temperature depend on the purpose of the experiment and cell type, and so will need to be optimized experimentally. Cells are washed in PBS with culture medium to remove extracellular remaining dye. Cells are observed under microscope or used for confocal microscopy, FACS or spectrofluorimetry analysis. Zinc is the second most abundant transition metal in the body and it is essential as catalytic, structural and regulatory ion. Zinc ions are involved in homeostasis, immune responses, oxidative stress, apoptosis and aging. Zinc has been proposed to function as a conventional neurotransmitter for the presynaptic neuron and as a transmembrane signal to traverse the postsynaptic neuron. Aberrant zinc metabolism is associated with many neurological diseases including Alzheimer's disease, Parkinson's disease and epilepsy. The most suitable technique for in vivo monitoring of zinc has been proven to be fluorescence imaging.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21261	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Zinquin AM *UltraPure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	218.112 µL	1.091 mL	2.181 mL	10.906 mL	21.811 mL
5 mM	43.622 µL	218.112 µL	436.224 µL	2.181 mL	4.362 mL
10 mM	21.811 µL	109.056 µL	218.112 µL	1.091 mL	2.181 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	458.48 g/mol	=	mL	x	mM	=	mmol

References

View all 8 references: Citation Explorer

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment

Authors:

Colvin RA, Laskowski M, Fontaine CP.

Journal:

Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin

Authors:

Jansen S, Arning J, Dulcks T, Beyersmann D.

Journal:

Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes

Authors:

Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.

Journal:

J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments

Authors:

Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.

Journal:

Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester

Authors:

Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.

Journal:

J Org Chem (2000): 8204

Physical properties

Molecular weight	458.48
Solvent	DMSO

Spectral properties

Excitation (nm)	355
Emission (nm)	491

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	DAPI
Emission	DAPI
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	360
Emission	480
Cutoff	455
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025