Zinquin ethyl ester *UltraPure grade*

Zinquin ethyl ester

UltraPure grade

Zinquin ethyl ester is a lipophilic, zinc-sensitive, cell-permeable fluorescent probe. It is retained in living cells because the ethyl ester is cleaved by cytosolic esterase to give Zinquin that carry a negative charge, preventing its efflux across the plasma membrane. Zinquin fluorescent probes may be loaded into cells by in the culture medium containing 5-40 uM Zinquin ethyl ester in PBS with calcium and magnesium (or in culture medium). Cells are normally incubated with the Zinquin ethyl ester for 15-30 minutes at 37 °C. Exact loading concentration, time and temperature depend on the purpose of the experiment and cell type, and so will need to be optimized experimentally. Cells are washed in PBS with culture medium to remove extracellular remaining dye. Cells are observed under microscope or used for confocal microscopy, FACS or spectrofluorimetry analysis. Zinc is the second most abundant transition metal in the body and it is essential as catalytic, structural and regulatory ion. Zinc ions are involved in homeostasis, immune responses, oxidative stress, apoptosis and aging. Zinc has been proposed to function as a conventional neurotransmitter for the presynaptic neuron and as a transmembrane signal to traverse the postsynaptic neuron. Aberrant zinc metabolism is associated with many neurological diseases including Alzheimer's disease, Parkinson's disease and epilepsy. The most suitable technique for in vivo monitoring of zinc has been proven to be fluorescence imaging.

Catalog	Size	Price	Quantity
21253	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Zinquin ethyl ester *UltraPure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	241.272 µL	1.206 mL	2.413 mL	12.064 mL	24.127 mL
5 mM	48.254 µL	241.272 µL	482.544 µL	2.413 mL	4.825 mL
10 mM	24.127 µL	120.636 µL	241.272 µL	1.206 mL	2.413 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	414.47 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Zinc deficiency triggers ribosome remodeling to reverse biofilm azole resistance in Candida albicans

Authors:

Wang, Yingzhe and Ye, Shigan and Deng, Yuan and Huang, Yingting and Zhu, Xiaoliang

Oyster-Derived Zinc-Binding Peptide Modified by Plastein Reaction via Zinc Chelation Promotes the Intestinal Absorption of Zinc

Authors:

Li, Jianpeng and Gong, Chen and Wang, Zaiyang and Gao, Ruichang and Ren, Jiaoyan and Zhou, Xiaodong and Wang, Haiyan and Xu, He and Xiao, Feng and Cao, Yuhui and others, undefined

Journal:

Marine drugs (2019): 341

References

View all 8 references: Citation Explorer

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment

Authors:

Colvin RA, Laskowski M, Fontaine CP.

Journal:

Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin

Authors:

Jansen S, Arning J, Dulcks T, Beyersmann D.

Journal:

Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes

Authors:

Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.

Journal:

J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments

Authors:

Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.

Journal:

Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester

Authors:

Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.

Journal:

J Org Chem (2000): 8204

Physical properties

Molecular weight	414.47
Solvent	DMSO

Spectral properties

Excitation (nm)	355
Emission (nm)	491

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	181530-09-6

Instrument settings

Flow cytometer
Excitation	355 nm laser
Emission	515/30 nm filter

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 21, 2025