AAT Bioquest

AssayWise Letters 2012. Vol. 1(1)

Multiplex Apoptosis and Necrosis
AAT Bioquest offers Cell Meter™ Apoptotic and Necrotic Detection Kits as a set of tools for monitoring cell viability. Our Cell Meter™ detection kits are optimized to simultaneously detect cell apoptosis, necrosis and healthy cells with a flow cytometer or fluorescence microscope.

Mulitplex Caspase Activities
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (Cat. #22797) is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation

Cal-520™, the Best Green Fluorescent Ca2+ Dye
Cal-520™ AM is our newest fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellu-lar retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cal-520™ provides a robust homogeneous fluorescence-based assay tool

The Rapid & Sensitive Detection of NAD, NADH, NADP & NADPH Using Amplite™ Assay Kits
NAD, NADH, NADP and NADPH are important cofactors in cells. AAT Bioquest Amplite™ NAD(P)/NAD(P)H assay kits have very low background since they are performed in a longer wavelength range that considerably reduces the interference resulted from biological samples. They have demonstrated high selectivity

Quantify Maleimide by Absorbance or Fluorescence
Our Amplite™ Colorimetric Maleimide Assay Kit quantifies ma-leimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining un-reacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M-1cm-1. The amount of maleimide is

Fluo-8®, the Brightest Green Ca2+ Indicators
Fluo-8® has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Fluo-8® AM only requires room temperature while Fluo-3 AM and Fluo-4 AM require 37 °C cell loading.

Quantify cAMP by Absorbance or Fluorescence
Cyclic adenosine monophosphate (cAMP) is an important second messenger in many biological processes. cAMP is derived from adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent pathway. cAMP is synthesised from

Optimized Fluorescence Labeling Solutions
Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies (including live cells) with exquisite sensitivity and selectivity. In general, the preferred bioconjugates should have high fluorescence quantum yields and retain the biological activities

Monitor Protease Contamination with Amplite™ Fluorimetric Assay Kits
Protease assays are widely used for the investigation of protease inhibitors and de-tection of protease activities.Our Amplite™ Universal Fluorimetric Protease Activity Assay Kits are an ideal choice to perform routine assays for the isolation of proteases, or for identifying the presence of

Detect Thiol with Amplite™ Assay Kits
The detection and measurement of free thiol (such as free cyste-ine, glutathione, and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.Our Amplite™ Fluorimetric Thiol Qutitation Assay Kit provides an ultrasensitive

Detect Calcium by Absorbance or Fluorescence
Calcium is essential for all living organisms, particularly in cell physiology. Calcium also plays an important role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion.Amplite™ Fluorimetric Calcium Quantitation