Bradford Stock Solution Preparation and Recipe
To prepare L of Bradford Stock Solution:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
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Component | Amount | Concentration |
Ethanol (95%) (mw: 46.07 g/mol) | 0.333 L | 31.67 % |
Phosphoric acid (88%) (mw: 98.00 g/mol) | 0.667 L | 58.67 % |
Coomassie Brilliant Blue (mw: 825.97 g/mol) | 1.16 g | 1.41 M |
- Prepare 0 mL of distilled water in a suitable container.
- Add 0.333 L of Ethanol (95%) to the solution.
- Add 0.667 L of Phosphoric acid (88%) to the solution.
- Add 1.16 g of Coomassie Brilliant Blue to the solution.
- Add distilled water until the volume is 1 L.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Bradford Stock Solution Preparation and Recipe." AAT Bioquest, Inc., 24 Sep. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bradford-stock-solution. | |
APA | AAT Bioquest, Inc. (2023, September 24). Quest Calculate™ Bradford Stock Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bradford-stock-solution. | |
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