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Bradford Stock Solution Preparation and Recipe

To prepare  L of Bradford Stock Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Ethanol (95%) (mw: 46.07 g/mol)0.333 L31.67 %
Phosphoric acid (88%) (mw: 98.00 g/mol)0.667 L58.67 %
Coomassie Brilliant Blue (mw: 825.97 g/mol)1.16 g1.41 M
  1. Prepare 0 mL of distilled water in a suitable container.
  2. Add 0.333 L of Ethanol (95%) to the solution.
  3. Add 0.667 L of Phosphoric acid (88%) to the solution.
  4. Add 1.16 g of Coomassie Brilliant Blue to the solution.
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
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Cell/Culture/Growth Media
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This online tool may be cited as follows


"Quest Calculate™ Bradford Stock Solution Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Bradford Stock Solution Preparation and Recipe. AAT Bioquest.