Bradford Stock Solution Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
Ethanol (95%) (mw: 46.07 g/mol)	0.333 L	31.67 %
Phosphoric acid (88%) (mw: 98.00 g/mol)	0.667 L	58.67 %
Coomassie Brilliant Blue (mw: 825.97 g/mol)	1.16 g	1.41 M

1. Prepare 0 mL of distilled water in a suitable container.
2. Add 0.333 L of Ethanol (95%) to the solution.
3. Add 0.667 L of Phosphoric acid (88%) to the solution.
4. Add 1.16 g of Coomassie Brilliant Blue to the solution.
5. Add distilled water until the volume is 1 L.

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