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DMEM/F-12 Recipe
Recipe Table
To prepareL of DMEM/F-12:
Change the value in the textbox above to scale the formulation volume
Comparison Table
| *Quantities in mg/L | Biotin | Calcium Chloride (CaCl2) (anhyd.) | Choline chloride | Cupric sulfate (CuSO4-5H2O) | D-Calcium pantothenate | D-Glucose (Dextrose) | Ferric Nitrate (Fe(NO3)3-9H2O) | Ferric sulfate (FeSO4-7H2O) | Folic acid | Glycine | Hypoxanthine Na | i-Inositol | L-Alanine | L-Arginine hydrochloride | L-Asparagine-H2O | L-Aspartic acid | L-Cysteine hydrochloride-H2O | L-Cystine 2HCl | L-Glutamic acid | L-Glutamine | L-Histidine hydrochloride-H2O | L-Isoleucine | L-Leucine | L-Lysine hydrochloride | L-Methionine | L-Phenylalanine | L-Proline | L-Serine | L-Threonine | L-Tryptophan | L-Tyrosine disodium salt dihydrate | L-Valine | Linoleic acid | Lipoic acid | Magnesium chloride (anhyd.) | Magnesium Sulfate (MgSO4) (anhyd.) | Niacinamide | Phenol Red | Potassium Chloride (KCl) | Putrescine 2HCl | Pyridoxine hydrochloride | Riboflavin | Sodium Bicarbonate (NaHCO3) | Sodium Chloride (NaCl) | Sodium phosphate dibasic (Na2HPO4) (anhyd.) | Sodium Phosphate monobasic (NaH2PO4-H2O) | Sodium pyruvate | Thiamine hydrochloride | Thymidine | Vitamin B12 | Zinc sulfate (ZnSO4-7H2O) |
| 0.0035 | 116.6 | 8.98 | 0.0013 | 2.24 | 3151 | 0.05 | 0.417 | 2.65 | 18.75 | 2.39 | 12.6 | 4.45 | 147.5 | 7.5 | 6.65 | 17.56 | 31.29 | 7.35 | 365 | 31.48 | 54.47 | 59.05 | 91.25 | 17.24 | 35.48 | 17.25 | 26.25 | 53.45 | 9.02 | 55.79 | 52.85 | 0.042 | 0.105 | 28.64 | 48.84 | 2.02 | 8.1 | 311.8 | 0.081 | 2.013 | 0.219 | 2438 | 6995.5 | 71.02 | 62.5 | 55 | 2.17 | 0.365 | 0.68 | 0.432 |
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References
This online resource may be cited as follows
MLA | "Quest Calculate™ DMEM/F-12 Recipe." AAT Bioquest, Inc., 8 Oct. 2025, https://www.aatbio.com/resources/cell-media-formulations/dmem-f-12-formulation-composition-recipe-and-preparation. | |
APA | AAT Bioquest, Inc. (2025, October 8). Quest Calculate™ DMEM/F-12 Recipe. AAT Bioquest. https://www.aatbio.com/resources/cell-media-formulations/dmem-f-12-formulation-composition-recipe-and-preparation. | |
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