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RPMI 1640 Medium Recipe
Recipe Table
To prepareL of RPMI 1640 Medium:
Change the value in the textbox above to scale the formulation volume
Comparison Table
| *Quantities in mg/L | Biotin | Calcium nitrate (Ca(NO3)2-4H2O) | Choline chloride | D-Calcium pantothenate | D-Glucose (Dextrose) | Folic acid | Glutathione (reduced) | Glycine | i-Inositol | L-Arginine | L-Asparagine | L-Aspartic acid | L-Cystine 2HCl | L-Glutamic acid | L-Glutamine | L-Histidine | L-Hydroxyproline | L-Isoleucine | L-Leucine | L-Lysine hydrochloride | L-Methionine | L-Phenylalanine | L-Proline | L-Serine | L-Threonine | L-Tryptophan | L-Tyrosine disodium salt dihydrate | L-Valine | Magnesium Sulfate (MgSO4) (anhyd.) | Niacinamide | Para-aminobenzoic acid | Phenol Red | Potassium Chloride (KCl) | Pyridoxine hydrochloride | Riboflavin | Sodium Bicarbonate (NaHCO3) | Sodium Chloride (NaCl) | Sodium phosphate dibasic (Na2HPO4) (anhyd.) | Thiamine hydrochloride | Vitamin B12 |
| 0.2 | 100 | 3 | 0.25 | 2000 | 1 | 1 | 10 | 35 | 200 | 50 | 20 | 65 | 20 | 300 | 15 | 20 | 50 | 50 | 40 | 15 | 15 | 20 | 30 | 20 | 5 | 29 | 20 | 48.84 | 1 | 1 | 5 | 400 | 1 | 0.2 | 2000 | 6000 | 800 | 1 | 0.005 |
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References
This online resource may be cited as follows
MLA | "Quest Calculate™ RPMI 1640 Medium Recipe." AAT Bioquest, Inc., 16 Oct. 2025, https://www.aatbio.com/resources/cell-media-formulations/rpmi-1640-medium-formulation-composition-recipe-and-preparation. | |
APA | AAT Bioquest, Inc. (2025, October 16). Quest Calculate™ RPMI 1640 Medium Recipe. AAT Bioquest. https://www.aatbio.com/resources/cell-media-formulations/rpmi-1640-medium-formulation-composition-recipe-and-preparation. | |
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