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Extinction Coefficient [Methylene Blue]
The molar extinction coefficient (ε) for Methylene Blue is
To request additional information and datasets for Methylene Blue, please contact info@aatbio.com.
Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS.
Search molar extinction coefficient (ε)
Select a compound from the list below to view molar extinction coefficient (ε) data. Enter keywords into the search box to filter the list.
1,8-ANS
1-ANS
2-Aminoacridone
3-Cyano-7-hydroxycoumarin
6-JOE
6-NED
7-AAD (7-Aminoactinomycin D)
7-Hydroxy-4-methylcoumarin (4-Methylumbelliferone)
AATOM™ 390
AATOM™ 425
AATOM™ 465
AATOM™ 488
AATOM™ 495
AATOM™ 514
AATOM™ 520
AATOM™ 532
AATOM™ 540Q
AATOM™ 550
AATOM™ 565
AATOM™ 580Q
AATOM™ 590
AATOM™ 594
AATOM™ 610
AATOM™ 612Q
AATOM™ 620
AATOM™ 633
AATOM™ 647
AATOM™ 647N
AATOM™ 655
AATOM™ 665
AATOM™ 680
AATOM™ 700
AATOM™ 725
AATOM™ 740
AATOM™ MB2
AATOM™ Oxa12
AATOM™ Rho101
AATOM™ Rho11
AATOM™ Rho12
AATOM™ Rho13
AATOM™ Rho14
AATOM™ Rho3B
AATOM™ Rho6G
AATOM™ Thio12
ABY
AFC (7-Amino-4-trifluoromethylcoumarin)
AMCA
APC (Allophycocyanin)
APC (Allophycocyanin)-Alexa Fluor 750
APC (Allophycocyanin)-Cy5.5
APC (Allophycocyanin)-Cy7
APC (Allophycocyanin)-eFluor 780
APC (Allophycocyanin)-iFluor 700
APC (Allophycocyanin)-iFluor 750
APC-H7
APC-XFD700
APC-XFD750
APC-iFluor 720
APC-iFluor 740
APC-iFluor 770
APC-iFluor 780
APC-iFluor™ 710
APC-iFluor™ 750
APC-iFluor™ 800
APTS
Acridine Orange
Alexa Fluor 350
Alexa Fluor 405
Alexa Fluor 430
Alexa Fluor 488
Alexa Fluor 514
Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 555
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 610
Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
Alexa Fluor 680-R-phycoerythrin
Alexa Fluor 700
Alexa Fluor 750
Alexa Fluor 790
AmCyan
Anthracene
Atto 390
Atto 425
Atto 465
Atto 488
Atto 495
Atto 514
Atto 520
Atto 532
Atto 540Q
Atto 550
Atto 565
Atto 580Q
Atto 590
Atto 594
Atto 610
Atto 612Q
Atto 620
Atto 633
Atto 647
Atto 647N
Atto 655
Atto 665
Atto 680
Atto 700
Atto 725
Atto 740
Atto MB2
Atto Oxa12
Atto Rho101
Atto Rho11
Atto Rho12
Atto Rho13
Atto Rho14
Atto Rho3B
Atto Rho6G
Atto Thio12
Auramine O
AzoDye-3
B-phycoerythrin
BCECF
BD Horizon PE (Phycoerythrin)-CF594
BD Horizon V450
BD Horizon V500
BHQ-1
BHQ-2
BO-PRO-1
BO-PRO-3
BOBO-1
BOBO-3
BODIPY 500/510
BODIPY 581/591
BODIPY 630/650
BODIPY 650/665
BODIPY FL
BODIPY R6G
BODIPY TMR
BODIPY TR
BODIPY-Phenyl
BODIPY-TMSCC
Biotin
Bodi Fluor 488
Bodi Fluor R6G
CAL Fluor Red 610
CM-DiD
CM-DiI
CR110 (Carboxyrhodamine 110)
CR6G (Carboxyrhodamine 6G)
CYTOXite™ Green
CYTOXite™ Red
Cal-590
Cal-590 Dextran
Calcein
Calcium Orange
California Red
Cascade Blue
Cascade Yellow
Cerulean (CFP variant)
Citrine
Coumarin 1
Coumarin 30
Coumarin 314
Coumarin 343
Coumarin 6
Cresyl Violet
Cy2
Cy3 (Cyanine-3)
Cy3.5 (Cyanine-3.5)
Cy3B
Cy3B Acid
Cy5 (Cyanine-5)
Cy5.5 (Cyanine-5.5)
Cy5.5NS
Cy7 (Cyanine-7)
Cy7.5 (Cyanine-7.5)
Cyber Orange
Cyluc1
D-Aminoluciferin
DAF-FM
DAPI (4,6-Diamidino-2-phenylindole)
DCF
DDAO
DRAQ5
DY-547
DY-555
DY-615
DY-630
DY-631
DY-633
DY-635
DY-636
DY-647
DY-650
DY-651
DY-675
DY-676
DY-680
DY-681
DY-700
DY-701
DY-730
DY-731
DY-750
DY-751
DY-776
DY-782
Dansyl
Dapoxyl
Di-4-ANEPPS
Di-8-ANEPPS
DiD
DiI
DiR
DiTO™-1
DiTO™-3
DiYO™-1
DiYO™-3
DsRed
DsRed Express
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800
EBFP2
ECFP
EDANS
EGFP
EVOblue-30
EYFP
Eosin
Ethidium homodimer
Ethyl-p-Dimethylaminobenzoate
FAM (Carboxyfluorescein)
FITC (Fluorescein-5-isothiocyanate)
FITC-xtra
FM 1-43
FM 4-64
Fluo-3
Fluo-4
Fluo-8
Fluorescein
Folic Acid
Glutite Red 670
HEX
Hoechst 33258
ICG (Indocyanine green)
ICG Xtra-OSu
Indo-1
JC-1
JOE (Carboxy-4,5-dichloro-2,7-dimethoxyfluorescein)
JOJO-1
JUN
LOLO-1
Li-Cor IRDye 800CW
Lucifer Yellow
MP650
Magnesium Green
Magnesium Orange
Malachite green
Marina Blue
Merocyanine 540
MitoLite Green FM
MitoTracker Green FM
NBD (Nitrobenzofurazan)
Naphthalene
Nile Blue
Nile Red
NirFP
Oregon Green 488
Oregon Green 514
Oxazine 1
Oxazine 170
PE (phycoerythrin)
PE (phycoerythrin)-Alexa Fluor 610
PE (phycoerythrin)-Alexa Fluor 647
PE (phycoerythrin)-Alexa Fluor 680
PE (phycoerythrin)-Alexa Fluor 700
PE (phycoerythrin)-Cy5
PE (phycoerythrin)-Cy5.5
PE (phycoerythrin)-Cy7
PE (phycoerythrin)-Texas Red
PE (phycoerythrin)-XFD 610
PE (phycoerythrin)-XFD 647
PE (phycoerythrin)-XFD 680
PE (phycoerythrin)-XFD 700
PE (phycoerythrin)-eFluor 610
PE (phycoerythrin)-iFluor 647
PE (phycoerythrin)-iFluor-750
PE-iFluor 720
PE-iFluor 740
PE-iFluor 770
PE-iFluor™ 594
PE-iFluor™ 597
PE-iFluor™ 610
PE-iFluor™ 660
PE-iFluor™ 700
PE-iFluor™ 710
PE-iFluor™ 780
PO-PRO-1
PO-PRO-3
POPO-1
POPO-3
POPOP
PPO
PacBlue
Pacific Blue
Pacific Orange
PerCP (Peridinin chlorophyll)
PerCP (Peridinin chlorophyll)-Cy5.5
PerCP (Peridinin chlorophyll)-eFluor 710
PerCP-Cy7
PerCP-iFluor 710
PerCP-iFluor700
PerCP-iFluor780
PicoGreen reagent
Proflavin
PromoFluor-350
PromoFluor-405
PromoFluor-415
PromoFluor-488
PromoFluor-488 Premium
PromoFluor-488LSS
PromoFluor-500LSS
PromoFluor-505
PromoFluor-510LSS
PromoFluor-514LSS
PromoFluor-520LSS
PromoFluor-532
PromoFluor-546
PromoFluor-555
PromoFluor-590
PromoFluor-610
PromoFluor-633
PromoFluor-647
PromoFluor-670
PromoFluor-680
PromoFluor-700
PromoFluor-750
PromoFluor-770
PromoFluor-780
Propidium iodide
Protonex™ Green 500
QSY 21
QSY 35
QSY 7
QSY 9
Qdot 525
Qdot 565
Qdot 585
Qdot 605
Qdot 625
Qdot 655
Qdot 705
Qdot 800
Quasar 570
Quasar 670
Quasar 705
RH 795
ROX (carboxy-X-rhodamine)
Resorufin
Rhodamine 110
Rhodamine 123
Rhodamine 6G
Rhodamine B
Rhodamine Green
Rhodamine Red
Rhodamine Red-X
Riboflavin
Rose Bengal
SYTO 11
SYTO 13
SYTO 17
SYTO 45
SYTOX Blue
SYTOX Green
SYTOX Orange
Squarylium dye III
Sulforhodamine 101
TAMRA (Carboxytetramethylrhodamine)
TET (Carboxy-2,4,7,7-tetrachlorofluorescein)
TF1 (Tide Fluor 1)
TF1-dUTP
TF2 (Tide Fluor 2)
TF2WS (Tide Fluor 2WS)
TF3 (Tide Fluor 3)
TF3 CEP
TF3WS (Tide Fluor 3WS)
TF4 (Tide Fluor 4)
TF5WS (Tide Fluor 5WS)
TF6WS (Tide Fluor 6WS)
TF7WS (Tide Fluor 7WS)
TF8WS (Tide Fluor 8WS)
TMP
TO-PRO-1
TO-PRO-3
TOTO-1
TOTO-3
TQ1 (Tide Quencher™ 1)
TQ2 (Tide Quencher™ 2)
TQ2WS (Tide Quencher™ 2WS)
TQ3 (Tide Quencher™ 3)
TQ3WS (Tide Quencher™ 3WS)
TQ4 (Tide Quencher™ 4)
TQ4WS (Tide Quencher™ 4WS)
TQ5WS (Tide Quencher™ 5WS)
TQ6WS (Tide Quencher™ 6WS)
TQ7.1 (Tide Quencher™ 7.1)
TQ7WS (Tide Quencher™ 7WS)
TRITC (Tetramethylrhodamine-isothiocyanate)
TUNELyte Red
TWO-PRO™ 1
TWO-PRO™ 3
TagBFP
Texas Red
Texas Red-X
Texas Red-X, ortho
XFD350 Alexa Fluor® 350 Equivalent
XFD405 Alexa Fluor® 405 Equivalent
XFD430 Alexa Fluor® 430 Equivalent
XFD488 Alexa Fluor® 488 Equivalent
XFD500 Alexa Fluor® 500 Equivalent
XFD514 Alexa Fluor® 514 Equivalent
XFD532 Alexa Fluor® 532 Equivalent
XFD546 Alexa Fluor® 546 Equivalent
XFD555 Alexa Fluor® 555 Equivalent
XFD568 Alexa Fluor® 568 Equivalent
XFD594 Alexa Fluor® 594 Equivalent
XFD610 Alexa Fluor® 610 Equivalent
XFD633 Alexa Fluor® 633 Equivalent
XFD635 Alexa Fluor® 635 Equivalent
XFD647 Alexa Fluor® 647 Equivalent
XFD660 Alexa Fluor® 660 Equivalent
XFD680 Alexa Fluor® 680 Equivalent
XFD700 Alexa Fluor® 700 Equivalent
XFD750 Alexa Fluor® 750 Equivalent
XFD790 Alexa Fluor® 790 Equivalent
YFP
YO-PRO-1
YO-PRO-3
YOYO-1
YOYO-3
ZnOEP
ZnTMP
iFluor 597
iFluor 350
iFluor 405
iFluor 430
iFluor 440
iFluor 450
iFluor 488
iFluor 500
iFluor 514
iFluor 532
iFluor 546
iFluor 555
iFluor 560
iFluor 568
iFluor 570
iFluor 594
iFluor 610
iFluor 633
iFluor 647
iFluor 660
iFluor 665
iFluor 670
iFluor 680
iFluor 690
iFluor 710
iFluor 740
iFluor 750
iFluor 770
iFluor 780
iFluor 790
iFluor 800
iFluor 810
iFluor 820
iFluor 840
iFluor 860
iFluor IRD 800 CW
iFluor™ 460
iFluor™ 700
iFluor™ 720
iFluor™ A7
iFluor™ Ultra 594
iFluor™ Ultra 647
iFluor™ Ultra 750
mCherry
mFluor Blue 583
mFluor Blue 585
mFluor Blue 659
mFluor™ Blue 570
mFluor™ Blue 580
mFluor™ Blue 590
mFluor™ Blue 615
mFluor™ Blue 620
mFluor™ Blue 630
mFluor™ Blue 660
mFluor™ Green 615
mFluor™ Green 620
mFluor™ Green 630
mFluor™ Red 700
mFluor™ Red 780
mFluor™ UV375
mFluor™ UV405
mFluor™ UV420
mFluor™ UV455
mFluor™ UV460
mFluor™ UV520
mFluor™ UV540
mFluor™ UV595
mFluor™ UV610
mFluor™ UV780
mFluor™ UVA550
mFluor™ UVB 370
mFluor™ UVB 460
mFluor™ Violet 420
mFluor™ Violet 430
mFluor™ Violet 450
mFluor™ Violet 480
mFluor™ Violet 500
mFluor™ Violet 505
mFluor™ Violet 510
mFluor™ Violet 530
mFluor™ Violet 540
mFluor™ Violet 545
mFluor™ Violet 550
mFluor™ Violet 575 SE
mFluor™ Violet 590
mFluor™ Violet 610
mFluor™ Yellow 630
mKO
mKate2
mOrange
mPlum
mRaspberry
mTFP1
mTangerine
mWasabi
trFluor EU
trFluor EU-Cryptate
xtraFluor™ Violet 420
zFluor 635
References
This online resource may be cited as follows
MLA | "Quest Database™ Extinction Coefficient [Methylene Blue]." AAT Bioquest, Inc., 4 Apr. 2026, https://www.aatbio.com/resources/extinction-coefficient/methylene_blue. | |
APA | AAT Bioquest, Inc. (2026, April 4). Quest Database™ Extinction Coefficient [Methylene Blue]. AAT Bioquest. https://www.aatbio.com/resources/extinction-coefficient/methylene_blue. | |
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