Extinction Coefficient [Nile Blue]

The molar extinction coefficient (ε) for Nile Blue is 76800 cm^-1M^-1 in EtOH

To request additional information and datasets for Nile Blue, please contact info@aatbio.com.

Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH₂O or PBS.

Nile Blue is spectrally similar to DiD, DiIC12(5)-DS, DiIC18(5)-DS, DiIC1(5) iodide

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Select a compound from the list below to view molar extinction coefficient (ε) data. Enter keywords into the search box to filter the list.

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1,8-ANS

1-ANS

2-Aminoacridone

3-Cyano-7-hydroxycoumarin

6-JOE

6-NED

7-AAD (7-Aminoactinomycin D)

7-Hydroxy-4-methylcoumarin (4-Methylumbelliferone)

AATOM™ 390

AATOM™ 425

AATOM™ 465

AATOM™ 488

AATOM™ 495

AATOM™ 514

AATOM™ 520

AATOM™ 532

AATOM™ 540Q

AATOM™ 550

AATOM™ 565

AATOM™ 580Q

AATOM™ 590

AATOM™ 594

AATOM™ 610

AATOM™ 612Q

AATOM™ 620

AATOM™ 633

AATOM™ 647

AATOM™ 647N

AATOM™ 655

AATOM™ 665

AATOM™ 680

AATOM™ 700

AATOM™ 725

AATOM™ 740

AATOM™ MB2

AATOM™ Oxa12

AATOM™ Rho101

AATOM™ Rho11

AATOM™ Rho12

AATOM™ Rho13

AATOM™ Rho14

AATOM™ Rho3B

AATOM™ Rho6G

AATOM™ Thio12

ABY

AFC (7-Amino-4-trifluoromethylcoumarin)

AMCA

APC (Allophycocyanin)

APC (Allophycocyanin)-Alexa Fluor 750

APC (Allophycocyanin)-Cy5.5

APC (Allophycocyanin)-Cy7

APC (Allophycocyanin)-eFluor 780

APC (Allophycocyanin)-iFluor 700

APC (Allophycocyanin)-iFluor 750

APC-H7

APC-XFD700

APC-XFD750

APC-iFluor 720

APC-iFluor 740

APC-iFluor 770

APC-iFluor 780

APC-iFluor™ 710

APC-iFluor™ 750

APC-iFluor™ 800

APTS

Acridine Orange

Alexa Fluor 350

Alexa Fluor 405

Alexa Fluor 430

Alexa Fluor 488

Alexa Fluor 514

Alexa Fluor 532

Alexa Fluor 546

Alexa Fluor 555

Alexa Fluor 568

Alexa Fluor 594

Alexa Fluor 610

Alexa Fluor 633

Alexa Fluor 635

Alexa Fluor 647

Alexa Fluor 660

Alexa Fluor 680

Alexa Fluor 680-R-phycoerythrin

Alexa Fluor 700

Alexa Fluor 750

Alexa Fluor 790

AmCyan

Anthracene

Atto 390

Atto 425

Atto 465

Atto 488

Atto 495

Atto 514

Atto 520

Atto 532

Atto 540Q

Atto 550

Atto 565

Atto 580Q

Atto 590

Atto 594

Atto 610

Atto 612Q

Atto 620

Atto 633

Atto 647

Atto 647N

Atto 655

Atto 665

Atto 680

Atto 700

Atto 725

Atto 740

Atto MB2

Atto Oxa12

Atto Rho101

Atto Rho11

Atto Rho12

Atto Rho13

Atto Rho14

Atto Rho3B

Atto Rho6G

Atto Thio12

Auramine O

AzoDye-3

B-phycoerythrin

BCECF

BD Horizon PE (Phycoerythrin)-CF594

BD Horizon V450

BD Horizon V500

BHQ-1

BHQ-2

BO-PRO-1

BO-PRO-3

BOBO-1

BOBO-3

BODIPY 500/510

BODIPY 581/591

BODIPY 630/650

BODIPY 650/665

BODIPY FL

BODIPY R6G

BODIPY TMR

BODIPY TR

BODIPY-Phenyl

BODIPY-TMSCC

Bodi Fluor 488

Bodi Fluor R6G

CAL Fluor Red 610

CM-DiD

CM-DiI

CR110 (Carboxyrhodamine 110)

CR6G (Carboxyrhodamine 6G)

CYTOXite™ Green

CYTOXite™ Red

Cal-590

Cal-590 Dextran

Calcein

Calcium Orange

California Red

Cascade Blue

Cascade Yellow

Cerulean (CFP variant)

Citrine

Coumarin 1

Coumarin 30

Coumarin 314

Coumarin 343

Coumarin 6

Cresyl Violet

Cy2

Cy3 (Cyanine-3)

Cy3.5 (Cyanine-3.5)

Cy3B

Cy3B Acid

Cy5 (Cyanine-5)

Cy5.5 (Cyanine-5.5)

Cy5.5NS

Cy7 (Cyanine-7)

Cy7.5 (Cyanine-7.5)

Cyber Orange

Cyluc1

D-Aminoluciferin

DABCYL

DAF-FM

DAPI (4,6-Diamidino-2-phenylindole)

DCF

DDAO

DRAQ5

DY-547

DY-555

DY-615

DY-630

DY-631

DY-633

DY-635

DY-636

DY-647

DY-650

DY-651

DY-675

DY-676

DY-680

DY-681

DY-700

DY-701

DY-730

DY-731

DY-750

DY-751

DY-776

DY-782

Dansyl

Dapoxyl

Di-4-ANEPPS

Di-8-ANEPPS

DiD

DiI

DiR

DiSC3(5)

DiTO™-1

DiTO™-3

DiYO™-1

DiYO™-3

DsRed

DsRed Express

DyLight 350

DyLight 405

DyLight 488

DyLight 550

DyLight 594

DyLight 633

DyLight 650

DyLight 680

DyLight 755

DyLight 800

EBFP2

ECFP

EDANS

EGFP

EVOblue-30

EYFP

Eosin

Ethidium homodimer

Ethyl-p-Dimethylaminobenzoate

FAM (Carboxyfluorescein)

FITC (Fluorescein-5-isothiocyanate)

FITC-xtra

FM 1-43

FM 4-64

Fluo-3

Fluo-4

Fluo-8

Fluorescein

Folic Acid

Glutite Red 670

HEX

Hoechst 33258

ICG (Indocyanine green)

ICG Xtra-OSu

Indo-1

JC-1

JOE (Carboxy-4,5-dichloro-2,7-dimethoxyfluorescein)

JOJO-1

JUN

LOLO-1

Li-Cor IRDye 800CW

Lucifer Yellow

MP650

MagaDye 535

MagaDye 561

MagaDye 588

MagaDye 613

Magnesium Green

Magnesium Orange

Malachite green

Marina Blue

Merocyanine 540

MitoLite Green FM

MitoTracker Green FM

NBD (Nitrobenzofurazan)

Naphthalene

Nile Blue

Nile Red

NirFP

NitroLite™ Green

Oregon Green 488

Oregon Green 514

Oxazine 1

Oxazine 170

PE (phycoerythrin)

PE (phycoerythrin)-Alexa Fluor 610

PE (phycoerythrin)-Alexa Fluor 647

PE (phycoerythrin)-Alexa Fluor 680

PE (phycoerythrin)-Alexa Fluor 700

PE (phycoerythrin)-Cy5

PE (phycoerythrin)-Cy5.5

PE (phycoerythrin)-Cy7

PE (phycoerythrin)-Texas Red

PE (phycoerythrin)-XFD 610

PE (phycoerythrin)-XFD 647

PE (phycoerythrin)-XFD 680

PE (phycoerythrin)-XFD 700

PE (phycoerythrin)-eFluor 610

PE (phycoerythrin)-iFluor 647

PE (phycoerythrin)-iFluor-750

PE-iFluor 720

PE-iFluor 740

PE-iFluor 770

PE-iFluor™ 594

PE-iFluor™ 597

PE-iFluor™ 610

PE-iFluor™ 660

PE-iFluor™ 700

PE-iFluor™ 710

PE-iFluor™ 780

PO-PRO-1

PO-PRO-3

POPO-1

POPO-3

POPOP

PPO

PacBlue

Pacific Blue

Pacific Orange

PerCP (Peridinin chlorophyll)

PerCP (Peridinin chlorophyll)-Cy5.5

PerCP (Peridinin chlorophyll)-eFluor 710

PerCP-Cy7

PerCP-iFluor 710

PerCP-iFluor700

PerCP-iFluor780

PicoGreen reagent

Proflavin

PromoFluor-350

PromoFluor-405

PromoFluor-415

PromoFluor-488

PromoFluor-488 Premium

PromoFluor-488LSS

PromoFluor-500LSS

PromoFluor-505

PromoFluor-510LSS

PromoFluor-514LSS

PromoFluor-520LSS

PromoFluor-532

PromoFluor-546

PromoFluor-555

PromoFluor-590

PromoFluor-610

PromoFluor-633

PromoFluor-647

PromoFluor-670

PromoFluor-680

PromoFluor-700

PromoFluor-750

PromoFluor-770

PromoFluor-780

Propidium iodide

Protonex™ Green 500

QSY 21

QSY 35

QSY 7

QSY 9

Qdot 525

Qdot 565

Qdot 585

Qdot 605

Qdot 625

Qdot 655

Qdot 705

Qdot 800

Quasar 570

Quasar 670

Quasar 705

RH 795

ROX (carboxy-X-rhodamine)

ReadiView™ Biotin

Resorufin

Rhodamine 110

Rhodamine 123

Rhodamine 6G

Rhodamine B

Rhodamine Green

Rhodamine Red

Rhodamine Red-X

Riboflavin

Rose Bengal

SYTO 11

SYTO 13

SYTO 17

SYTO 45

SYTOX Blue

SYTOX Green

SYTOX Orange

Squarylium dye III

Sulforhodamine 101

TAMRA (Carboxytetramethylrhodamine)

TET (Carboxy-2,4,7,7-tetrachlorofluorescein)

TF1 (Tide Fluor 1)

TF1-dUTP

TF2 (Tide Fluor 2)

TF2WS (Tide Fluor 2WS)

TF3 (Tide Fluor 3)

TF3 CEP

TF3WS (Tide Fluor 3WS)

TF4 (Tide Fluor 4)

TF5WS (Tide Fluor 5WS)

TF6WS (Tide Fluor 6WS)

TF7WS (Tide Fluor 7WS)

TF8WS (Tide Fluor 8WS)

TMP

TO-PRO-1

TO-PRO-3

TOTO-1

TOTO-3

TQ1 (Tide Quencher™ 1)

TQ2 (Tide Quencher™ 2)

TQ2WS (Tide Quencher™ 2WS)

TQ3 (Tide Quencher™ 3)

TQ3HWS (Tide Quencher™ 3HWS)

TQ3WS (Tide Quencher™ 3WS)

TQ4 (Tide Quencher™ 4)

TQ4WS (Tide Quencher™ 4WS)

TQ5WS (Tide Quencher™ 5WS)

TQ6WS (Tide Quencher™ 6WS)

TQ7.1 (Tide Quencher™ 7.1)

TQ7WS (Tide Quencher™ 7WS)

TRITC (Tetramethylrhodamine-isothiocyanate)

TUNELyte Red

TWO-PRO™ 1

TWO-PRO™ 3

TagBFP

Texas Red

Texas Red-X

Texas Red-X, ortho

XFD350 Alexa Fluor® 350 Equivalent

XFD405 Alexa Fluor® 405 Equivalent

XFD430 Alexa Fluor® 430 Equivalent

XFD488 Alexa Fluor® 488 Equivalent

XFD500 Alexa Fluor® 500 Equivalent

XFD514 Alexa Fluor® 514 Equivalent

XFD532 Alexa Fluor® 532 Equivalent

XFD546 Alexa Fluor® 546 Equivalent

XFD555 Alexa Fluor® 555 Equivalent

XFD568 Alexa Fluor® 568 Equivalent

XFD594 Alexa Fluor® 594 Equivalent

XFD610 Alexa Fluor® 610 Equivalent

XFD633 Alexa Fluor® 633 Equivalent

XFD635 Alexa Fluor® 635 Equivalent

XFD647 Alexa Fluor® 647 Equivalent

XFD660 Alexa Fluor® 660 Equivalent

XFD680 Alexa Fluor® 680 Equivalent

XFD700 Alexa Fluor® 700 Equivalent

XFD750 Alexa Fluor® 750 Equivalent

XFD790 Alexa Fluor® 790 Equivalent

YFP

YO-PRO-1

YO-PRO-3

YOYO-1

YOYO-3

ZnOEP

ZnTMP

iFluor 597

iFluor 350

iFluor 405

iFluor 430

iFluor 440

iFluor 445

iFluor 450

iFluor 488

iFluor 500

iFluor 514

iFluor 532

iFluor 540

iFluor 546

iFluor 555

iFluor 560

iFluor 568

iFluor 570

iFluor 594

iFluor 610

iFluor 633

iFluor 647

iFluor 660

iFluor 665

iFluor 670

iFluor 680

iFluor 690

iFluor 710

iFluor 740

iFluor 750

iFluor 770

iFluor 780

iFluor 790

iFluor 800

iFluor 810

iFluor 820

iFluor 840

iFluor 860

iFluor IRD 800 CW

iFluor® 525

iFluor® 560 Plus

iFluor™ 460

iFluor™ 700

iFluor™ 720

iFluor™ A7

iFluor™ Ultra 594

iFluor™ Ultra 647

iFluor™ Ultra 750

mCherry

mFluor Blue 583

mFluor Blue 585

mFluor Blue 659

mFluor™ Blue 570

mFluor™ Blue 580

mFluor™ Blue 590

mFluor™ Blue 615

mFluor™ Blue 620

mFluor™ Blue 630

mFluor™ Blue 660

mFluor™ Green 615

mFluor™ Green 620

mFluor™ Green 630

mFluor™ Red 700

mFluor™ Red 780

mFluor™ UV375

mFluor™ UV405

mFluor™ UV420

mFluor™ UV455

mFluor™ UV460

mFluor™ UV520

mFluor™ UV540

mFluor™ UV595

mFluor™ UV610

mFluor™ UV780

mFluor™ UVA550

mFluor™ UVB 370

mFluor™ UVB 460

mFluor™ Violet 420

mFluor™ Violet 430

mFluor™ Violet 450

mFluor™ Violet 480

mFluor™ Violet 500

mFluor™ Violet 505

mFluor™ Violet 510

mFluor™ Violet 530

mFluor™ Violet 540

mFluor™ Violet 545

mFluor™ Violet 550

mFluor™ Violet 575 SE

mFluor™ Violet 590

mFluor™ Violet 610

mFluor™ Yellow 630

mKO

mKate2

mOrange

mPlum

mRaspberry

mTFP1

mTangerine

mWasabi

trFluor EU

trFluor EU-Cryptate

trFluor TB

xtraFluor™ Violet 420

zFluor 635

References

This online resource may be cited as follows

MLA	"Quest Database™ Extinction Coefficient [Nile Blue]." AAT Bioquest, Inc., 15 Sep. 2026, https://www.aatbio.com/resources/extinction-coefficient/nile_blue.
APA	AAT Bioquest, Inc. (2026, September 15). Quest Database™ Extinction Coefficient [Nile Blue]. AAT Bioquest. https://www.aatbio.com/resources/extinction-coefficient/nile_blue.
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