logo
AAT Bioquest

How do you determine DNA purity?

Posted February 4, 2020


Answer

DNA purity in a sample can be determined by measuring sample absorbance at 260 and 280 nm and determining their ratio, where the ratio of 1.7–2.0 indicates pure DNA sample. Contaminants which also absorb at 260 and 280 nm may cause overestimation of DNA in the sample.