How is IC50 calculated?

IC₅₀ of a drug is obtained by generating a dose-response curve and analyzing the drug-inhibitor interaction at different concentrations. Based on the plot of the dose-response curve, IC₅₀ values are derived for specific inhibitors by determining the concentration required to reduce 50% of the maximum response from the drug. Generally, for a fixed concentration of inhibitory substance, a higher inhibition indicates a lower drug response and hence, a smaller IC₅₀ value. For a quick solution, use our free online IC₅₀ calculator, Quest Graph™ IC₅₀ Calculator, which allows for an accurate and efficient approach to determining IC₅₀ values representative of your data set.