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How much BSA should I add to PCR?
Posted February 11, 2021
DNA and RNA
Physiological Probes
Bioconjugation
Answer
BSA (bovine serum albumin) is particularly useful to enhance the efficiency or specificity of PCR. The concentration depends on the template, but as a general rule, 0.01 µg/µl to 0.5 µg/ µl BSA (final concentration) can be used.
Additional resources
Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates
ReadiLink™ BSA Conjugation Kit
Polymerase Chain Reaction (PCR)
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