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Is PE Cy7 photosensitive?
Posted July 1, 2021
Answer
Yes, PE-Cy7 is highly photosensitive.
PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE) and cyanine dye Cy7. This bright dye is often used as a fluorescence-based indicator in flow cytometry for detecting low-abundance antigens. Its use is immunofluorescence microscopy is limited due to its rapid photobleaching property. The dye degrades rapidly when exposed to photobleaching, higher temperatures and paraformaldehyde fixation.
Additional resources
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)