AAT Bioquest

Is ethidium bromide visible under UV?

Posted December 1, 2021


Yes, ethidium bromide is visible under UV. Ethidium bromide (EtBr) is commonly used as a non-radioactive dye to visualize DNA in agarose gel electrophoresis experiments.

Ethidium bromide has UV absorbance maxima at 300 and 360 nm and an emission maximum at 590 nm. It fluoresces a red-orange color under UV light and with increased brightness after binding to dsDNA.

Additional resources

Mechanisms and pathways of ethidium bromide Fenton-like degradation by reusable magnetic nanocatalysts

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