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AAT Bioquest

What are the applications of Immunoblot?

Posted February 4, 2022


Answer

Immunoblot is an important technique in biochemistry. It is used for the qualitative detection of a single protein or a protein modification in a sample. This has a wide range of applications including:

  • Detecting the presence of phosphorylated proteins
  • Detecting tagged proteins
  • Determining changes in protein levels across various time points
  • Determining changes in protein levels across various treatment groups
  • Identifying truncated isoforms of proteins
Additional resources

Applications of western blot technique: From bench to bedside

Immunohistochemistry (IHC)