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What filters should I use for Apoptosis/Necrosis Assay Kit (Cat No. 22843)?

Posted July 28, 2020


Answer

The Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence* (Cat No. 22843) requires three filter sets for visualization. See Table 1 and 2 below.


Table 1. Flow cytometer channels required for Cat No. 22843.

Probe Excitation Laser (nm) Channel Channel Specification (nm) Cell Population
CytoCalcein™ Violet 450 405 Pacific Blue 450/40 Viable Cells
Nuclear Green™ DCS1 488 FITC 530/30 Necrotic Cells
Apopxin™ Deep Red 633 APC 660/20 Apoptotic Cells


Table 2. Fluorescence microscope filter sets required for Cat No. 22843.

Probe Excitation Filter Emission Filter Cell Population
CytoCalcein™ Violet 450 DAPI DAPI Viable Cells
Nuclear Green™ DCS1 FITC FITC Necrotic Cells
Apopxin™ Deep Red Cy5 Cy5 Apoptotic Cells
Additional resources

Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors*