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What filters should I use for Apoptosis/Necrosis Assay Kit (Cat No. 22843)?
Posted July 28, 2020
Answer
The Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence* (Cat No. 22843) requires three filter sets for visualization. See Table 1 and 2 below.
Table 1. Flow cytometer channels required for Cat No. 22843.
| Probe | Excitation Laser (nm) | Channel | Channel Specification (nm) | Cell Population |
| CytoCalcein™ Violet 450 | 405 | Pacific Blue | 450/40 | Viable Cells |
| Nuclear Green™ DCS1 | 488 | FITC | 530/30 | Necrotic Cells |
| Apopxin™ Deep Red | 633 | APC | 660/20 | Apoptotic Cells |
Table 2. Fluorescence microscope filter sets required for Cat No. 22843.
| Probe | Excitation Filter | Emission Filter | Cell Population |
| CytoCalcein™ Violet 450 | DAPI | DAPI | Viable Cells |
| Nuclear Green™ DCS1 | FITC | FITC | Necrotic Cells |
| Apopxin™ Deep Red | Cy5 | Cy5 | Apoptotic Cells |
Additional resources
Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors*