AAT Bioquest

What is IHC?

Posted February 25, 2020


Immunohistochemistry (IHC) is the most common method of immunostaining. It uses antibodies to selectively identify antigens in biological tissues. In order to visualize antibody-antigen binding, the antibody is typically labeled with reporter molecules. In the most common instance, the antibody is conjugated to an enzyme, such as horseradish peroxidase (HRP) that can catalyze a color-producing reaction with its respective substrate. The signal output is generally colorimetric. Another alternative is to label your antibody with a fluorophore, such as fluorescein, and image using a fluorescence microscope.