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AAT Bioquest

What is full spectrum flow cytometry?

Posted November 11, 2021


Answer

Full spectrum flow cytometry is a highly advanced technique that uses highly sensitive light detectors to capture the entire emission spectrum of every fluorescent molecule across a specified range of wavelengths.

Full spectrum flow cytometry is a more efficient way to measure fluorescence. It allows researchers to separate and distinguish fluorophores with very similar emission spectra, which is not possible using conventional flow cytometry methods. Full spectrum Cytometry offers more opportunities to expand panels and enhance detection, especially in cells that are highly autofluorescent.

Additional resources

Advantages of full spectrum flow cytometry

Spectral Flow Cytometry

Fluorescence Spectrum Viewer

mFluor™ Red 780 SE