What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?

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Accepted Answer

Below is a brief protocol for staining cells with MM 1-43:

Dissolve MM 1-43 in DMSO to make a 10 mg/mL stock solution.

Recommended storage temperature for stock solution is 4oC.

Dilute your stock solution to 100 &mu;g/mL in DMSO and then dilute with water before staining.

Your final stain concentration should be approximately 1-10 &mu;g/mL.  Make sure to prepare a fresh solution in water every time prior to use.

Incubate your sample for 15-30 minutes at room temperature, protected from light.
Rinse your sample gently with sterilized water.
Image your sample immediately after washing.

Ex/Em = ~510/626 nm

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