Below is a brief protocol for staining cells with MM 1-43:

1. Dissolve MM 1-43 in DMSO to make a 10 mg/mL stock solution.
   1. Recommended storage temperature for stock solution is 4^oC.
2. Dilute your stock solution to 100 µg/mL in DMSO and then dilute with water before staining.
   1. Your final stain concentration should be approximately 1-10 µg/mL.  Make sure to prepare a fresh solution in water every time prior to use.
3. Incubate your sample for 15-30 minutes at room temperature, protected from light.
4. Rinse your sample gently with sterilized water.
5. Image your sample immediately after washing.
   1. Ex/Em = ~510/626 nm