What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?
Posted April 19, 2018
Can you provide a protocol?
Answer
Below is a brief protocol for staining cells with MM 1-43:
- Dissolve MM 1-43 in DMSO to make a 10 mg/mL stock solution.
- Recommended storage temperature for stock solution is 4oC.
- Dilute your stock solution to 100 μg/mL in DMSO and then dilute with water before staining.
- Your final stain concentration should be approximately 1-10 μg/mL. Make sure to prepare a fresh solution in water every time prior to use.
- Incubate your sample for 15-30 minutes at room temperature, protected from light.
- Rinse your sample gently with sterilized water.
- Image your sample immediately after washing.
- Ex/Em = ~510/626 nm
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