What is the difference between ratiometric imaging and calcium imaging?
Posted July 23, 2021
Answer
Features of Ratiometric Imaging:
- Is a technique that involves measuring the intensities at two or more wavelengths of an excitation or emission spectrum in order to detect fluctuations in local environment
- Involves the use of a probe that is specifically sensitive to an environmental parameter such as pH, polarity, ion concentration or viscosity
- Is based on the use of a ratio between two fluorescence intensities
Features of Calcium Imaging:
- Is a microscopy technique used to optically trace and measure cellular calcium signals
- Involves the use of calcium indicators, which are fluorescent molecules that respond to the binding of calcium ions by fluorescence properties
- Is based on the sensitivity of the calcium indicators to fluctuations in specific environmental factors such as temperature, pressure, pH, ionic strength, and proximity of other fluorophore species
Additional resources
A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging