What is the molecular weight (mw) of pyruvate kinase?

228 kDa/114 kDa

Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. In terms of structure, there is both a tetrameric and dimeric form of pyruvate kinase. In its R-state conformation, namely with high binding affinity to PEP, the molecular weight of pyruvate kinase is 228 kDa and it exists as a tetramer of four equal subunits of molecular weight 57 kDa. In contrast, in its T-state conformation, namely with a low binding affinity to PEP, the molecular weight of pyruvate kinase is 114 kDa and it exists as a dimer of two equal subunits of molecular weight 57 kDa.