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What kinds of interactions are used in affinity chromatography?
Posted June 1, 2020
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification
Answer

Affinity chromatography separates biochemical mixtures based on the reversible and highly specific interactions such as the interactions between antibody and antigen, enzyme and substrate, receptor and ligand, or protein and nucleic acids. Other interactions that also commonly being used in affinity chromatography are:

  • Proteins A and G-immunoglobulin
  • Metal ions-poly fusion protein
  • Avidin-biotin
  • Lectin-polysaccharide
  • Nucleic acid-complementary base sequence
  • Poly A sequence-RNA containing poly U sequence
  • Hormone-receptor
  • Lectin-polysaccharide
Additional resources
Antibody development servicesUrh, M., Simpson, D., & Zhao, K. (2009). Affinity chromatography: general methods. In Methods in enzymology (Vol. 463, pp. 417-438). Academic Press.

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