Posted May 9, 2020
Answer
Ionophores A231187 or ionomycin can be used to stimulate intracellular calcium release. Thapsigargin (TG) and cyclopiazonic acid (CPA), which are both SERCA pump inhibitors, can be used to increase calcium levels as well.
Additional resources
Takami, M., Takahashi, N., Udagawa, N., Miyaura, C., Suda, K., Woo, J. T., ... & Suda, T. (2000). Intracellular calcium and protein kinase C mediate expression of receptor activator of nuclear factor-?B ligand and osteoprotegerin in osteoblasts. Endocrinology, 141(12), 4711-4719.
Chung, K. C., Sung, J. Y., Ahn, W., Rhim, H., Oh, T. H., Lee, M. G., & Ahn, Y. S. (2001). Intracellular calcium mobilization induces immediate early genepip92 via src and mitogen-activated protein kinase in immortalized hippocampal cells. Journal of Biological Chemistry, 276(3), 2132-2138.
Amplite™ Colorimetric Calcium Quantitation Kit *Blue Color*
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit
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