Why does a secondary antibody have to originate from a host species different than that of the primary antibody? | AAT Bioquest

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Why does a secondary antibody have to originate from a host species different than that of the primary antibody?

Posted April 24, 2020

Antibodies and Proteomics Bioconjugation Antibody and Protein Labeling Immunohistochemistry (IHC)Primary Antibodies Secondary Antibodies

Answer

A secondary antibody has to originate from a different host in order to be reactive against the primary antibody. This is because an antibody from the same host as another will not recognize the other antibody as foreign, and thus will not bind to it.

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iFluor® 594 goat anti-rabbit IgG (H+L) Secondary Antibodies

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