Why does trypan blue quench FITC or GFP in flow cytometry?
Posted May 18, 2020
Trypan blue absorbs light in the green-yellow region with a maximum absorbance wavelength around 590 nm in water. It can absorb the fluorescence emitted by green-emission fluorochromes or fluorescent proteins, thus quenching FITC or GRP in flow cytometry.
Trypan Blue, sodium salt *10 mM aqueous solution*
Graham, J. P., Rauf, M. A., Hisaindee, S., & Nawaz, M. (2013). Experimental and theoretical study of the spectral behavior of Trypan Blue in various solvents. Journal of Molecular Structure, 1040, 1–8. doi:10.1016/j.molstruc.2013.02.015
Shilova, O. N., Shilov, E. S., & Deyev, S. M. (2017). The effect of trypan blue treatment on autofluorescence of fixed cells. Cytometry Part A, 91(9), 917–925. doi:10.1002/cyto.a.23199