Can I use PCR primers for sequencing?
Posted July 13, 2022
Yes, you can use PCR primers for sequencing, provided that they meet these stipulations:
- The residual PCR primers must be removed from the reaction before submitting it for sequencing.
- The primers you are using must be compatible with the specific sequencing conditions.
PCR reactions can only be quantitated successfully with a very sensitive spectrophotometer. If you don’t have one, you will need to use an analytical gel to estimate the concentration.