AAT Bioquest

Can I use PCR primers for sequencing?

Posted July 13, 2022


Yes, you can use PCR primers for sequencing, provided that they meet these stipulations: 

  • The residual PCR primers must be removed from the reaction before submitting it for sequencing.
  • The primers you are using must be compatible with the specific sequencing conditions. 

PCR reactions can only be quantitated successfully with a very sensitive spectrophotometer. If you don’t have one, you will need to use an analytical gel to estimate the concentration. 

Additional resources

Primer design for RT-PCR

Polymerase Chain Reaction (PCR)

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*