How are FITC signals detected? | AAT Bioquest

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How are FITC signals detected?

Posted November 10, 2022

Antibodies and Proteomics Bioconjugation Chemical Reagents Antibody and Protein Labeling Classic Dyes Fluoresceins Immunohistochemistry (IHC)amine-reactive immunocytochemistry

Answer

FITC signals can be detected a couple of different ways. Direct detection is possible with the use of fluorescence microscopes. The indirect technique involves the use of an anti-FITC antibody that enables detection by amplifying FITC signals and/or eliminating the quenching of FITC fluorescence.

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