ATP assays have a wide range of applications. When choosing an ATP assay, the first and most important step is understanding the purpose for using the assay. In addition, your choice of ATP assay will also depend on the nature and number of samples being tested, the desired sensitivity, and the detection instruments and automated liquid handlers that are available. 

If you are using 3D cell culture or performing high-throughput screening, you’ll need to make sure that the assay you choose has been tested and optimized for those specific applications.