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How do I remove the RNA primers?
Posted February 17, 2023
DNA and RNAEnzymesPolymerase Chain Reaction (PCR)Polymerases
Answer
RNA primers are removed by the enzyme DNA polymerase I. The nicks that remain after the primers are replaced are sealed by enzyme DNA ligase. DNA fragments replace the RNA primers. 
Additional resources
Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells* Polymerase Chain Reaction (PCR) Helixyte™ Green *20X Aqueous PCR Solution*

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