AAT Bioquest

Is FITC hydrophobic?

Posted November 10, 2022


FITC is insoluble in water. It is generally supplied as a lyophilized solid and must be resuspended in an organic solvent, such as ethanol, DMSO (Dimethyl sulfoxide), or DMF (Dimethylformamide), before labeling proteins or any other amine-containing biomolecule. 

Additional resources

Dual FITC lateral flow immunoassay for sensitive detection of Escherichia coli O157:H7 in food samples

Fluorescein isothiocyanate (FITC)

5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*