Is FITC hydrophobic?

Posted November 10, 2022

Antibodies and Proteomics Bioconjugation Chemical Reagents Antibody and Protein Labeling Classic Dyes Flow Cytometry Reagents Fluoresceins Immunohistochemistry (IHC)Spectral Flow Cytometry immunocytochemistry

Answer

FITC is insoluble in water. It is generally supplied as a lyophilized solid and must be resuspended in an organic solvent, such as ethanol, DMSO (Dimethyl sulfoxide), or DMF (Dimethylformamide), before labeling proteins or any other amine-containing biomolecule.

Additional resources

Dual FITC lateral flow immunoassay for sensitive detection of Escherichia coli O157:H7 in food samples Fluorescein isothiocyanate (FITC) 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

What are the physical properties of FITC?Is FITC a fluorophore?What are the different isomers of FITC?How do I store FITC-dextran?What are the applications of TRITC-dextran?

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