What are the advantages of using immobilized enzymes over using free enzymes?
Posted February 22, 2024
Answer
Using immobilized enzymes over free enzymes offers several advantages including:
- Higher stability because enzyme molecules are protected by immobilizing matrix
- Immediate availability of enzymes for reusing multiple times, which allows for continuous processes
- Reactions are more controllable as enzymes can be removed during any stage of the reaction
- Lower purification and downstream processes as enzymes are absent in products
- Lower chances of product being contaminated with enzyme as the enzyme can be taken out from the product mixture
- Lower cost of production and separation from being able to reuse enzymes repetitively
Additional resources
Enzyme immobilization: an overview on techniques and support materials
Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*