There are various advantages of using serial dilution in microbiology.
Serial dilution helps to gradually decrease a high concentrate cell culture to a more manageable and practical level.
Counting the colonies obtained from these samples helps estimate the concentration of an unknown substance.
The known concentration solution is measured just once. As the concentration decreases with each step, the error margin in each subsequent standard decreases significantly.
Having evenly spaced standards enhances the reliability of the analysis results.
This method is also advantageous when the calibration standards need to cover a wide range of concentrations.