What are the applications of proteinase K?
Posted May 19, 2023
Proteinase K is used extensively in a wide range of molecular biology applications and next generation sequencing (NGS) and microarray technologies.
In molecular biology Proteinase K is used to:
- Digest proteins and remove contamination from nucleic acid preparations
- Inactivate nucleases DNases and RNases that might otherwise degrade the DNA or RNA sample during purification
- Obtain characteristic protein fragments to be used in determining the structure and function of proteins
- Isolate highly native and undamaged DNA or RNA by rapidly inactivating mammalian and microbial DNases and RNases
- Specifically modify cell surface proteins in order to analyze membrane structures for protein localization
- Digest tissue as an alternative option for preparing samples for quantitative analysis via liquid chromatography - tandem mass spectrometry
- Detect Bovine Spongiform Encephalopathy proteins, which are uniquely resistant to proteolytic degradation.
In next generation sequencing (NGS) and microarray technologies, Proteinase K is used to:
- Inactivate nucleases and purify nucleic acid during the extraction of DNA and RNA from bacteria, yeast, and plant cell and mammalian cell lysates
- Improve cloning efficiency of PCR products
- Inactivate enzyme cocktails in ribonuclease protection assays
- Prepare samples for quantifying DNA adduct levels using accelerator mass spectrometry
- Optimize RNA yields from primary breast tumors for microarray studies