What are the differences between dNTPs and ddNTPs?

Question

Accepted Answer

Basis of differentiation

dNTPs

ddNTPs

Definition

dNTPs are nucleotides which are building blocks of DNA (for DNA synthesis)

ddNTPs are specialized  nucleotides which are used in the Sanger Sequencing method

Presence of 3’OH group

Possess a 3’OH group in the deoxyribose sugar

Does not have a 3’OH group

DNA synthesis

Increases the length of the DNA sequence

It terminates the DNA sequence

Phosphodiester bond

Form phosphodiester bonds

Are not able to form phosphodiester bonds

Use in Sanger Sequencing

Is used during standard PCR procedures

Are used as chain terminations in Sanger Sequencing reactions

Basis of differentiation	dNTPs	ddNTPs
Definition	dNTPs are nucleotides which are building blocks of DNA (for DNA synthesis)	ddNTPs are specialized nucleotides which are used in the Sanger Sequencing method
Presence of 3’OH group	Possess a 3’OH group in the deoxyribose sugar	Does not have a 3’OH group
DNA synthesis	Increases the length of the DNA sequence	It terminates the DNA sequence
Phosphodiester bond	Form phosphodiester bonds	Are not able to form phosphodiester bonds
Use in Sanger Sequencing	Is used during standard PCR procedures	Are used as chain terminations in Sanger Sequencing reactions