Basis of differentiation | Primary cell culture | Cell line |
Definition | Primary cell culture is the growing and regulation of the specific cell type which has been removed from parental tissue | Is a cell culture derived from a single cell, composed of cells with identical genetic material |
Significance in vivo | Is an in vivo model | Is not an in vivo model, |
Lifespan | Has a limited lifespan | Has an unlimited lifespan in culture |
Donor characteristics | Exhibits donor characteristics | Does not exhibit any donor characteristics |
Relation to parent tissue | Has the same genetic features as the parent tissue | Has an altered genetic makeup compared to its parent tissue |
Passaging | Is unable to use passaging | Can be regulated by using passaging |
Isolation mechanism | Is isolated directly from its donor | Is isolated from primary cell culture through immortalization |