AAT Bioquest

What are the limitations of using ANEP dyes in membrane potential activity analysis?

Posted October 20, 2023


ANEP dyes are common fluorescent dyes that respond to electrical potential changes. They are ultra fast-responding, and can provide millisecond response time.  This dye method, however, suffers from a number of drawbacks, including a weak signal-to-noise ratio. Additionally, the magnitude of their potential-dependent fluorescence changes frequently, as this measurement is dependent upon membrane potential (MP), which can make analysis and true quantification tricky.

Additional resources

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Cell/Cytoplasmic Membrane Potential Activity & Analysis

Di-8-ANEPPS *CAS#: 157134-53-7*