AAT Bioquest

What are the physical properties of FITC?

Posted November 10, 2022


FITC (Fluorescein-5-isothiocyanate) is a yellow compound available in powder form. Its molecular weight is 389.4 grams per mole. 

FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. It emits a green light.

Additional resources

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Fluorescein isothiocyanate (FITC)

5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*