What are the physical properties of FITC? | AAT Bioquest

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What are the physical properties of FITC?

Posted November 10, 2022

Antibodies and Proteomics Bioconjugation Chemical Reagents Antibody and Protein Labeling Classic Dyes Flow Cytometry Reagents Fluoresceins Immunohistochemistry (IHC)Spectral Flow Cytometry immunocytochemistry

Answer

FITC (Fluorescein-5-isothiocyanate) is a yellow compound available in powder form. Its molecular weight is 389.4 grams per mole.

FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. It emits a green light.

Additional resources

Imaging FITC-dextran as a Reporter for Regulated Exocytosis Fluorescein isothiocyanate (FITC) 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

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