What are the steps for transfection with calcium phosphate?

Calcium phosphate transfection is a widely used technique for introducing foreign DNA plasmids into cells. Transfection with calcium phosphate follows these steps: 

• DNA is mixed with the transfection agent – calcium chloride. 
• The DNA and Calcium Chloride complex is added to phosphate buffer to generate nanoparticulate precipitates. Optimum pH range is between 6.9 and 7.4.
• The DNA and Calcium Chloride complex precipitates. 
• The precipitate is removed and dispersed in cell culture.
• Phagocytosis of the co-precipitate by the target cell.