Calcium phosphate transfection is a widely used technique for introducing foreign DNA plasmids into cells. Transfection with calcium phosphate follows these steps:
DNA is mixed with the transfection agent – calcium chloride.
The DNA and Calcium Chloride complex is added to phosphate buffer to generate nanoparticulate precipitates. Optimum pH range is between 6.9 and 7.4.
The DNA and Calcium Chloride complex precipitates.
The precipitate is removed and dispersed in cell culture.
Phagocytosis of the co-precipitate by the target cell.