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AAT Bioquest

What are the types of microarrays based on the probes used?

Posted August 16, 2023


Answer

There are many types of microarrays based on the probes used. Some of these microarrays include: DNA microarrays, MMChips, cellular microarrays, tissue microarrays, antibody microarrays, protein microarrays, phenotype arrays, and peptide microarrays. 

  1. DNA microarrays, also known as gene chips, measure DNA or use DNA as a part of its detection system. The four types of DNA microarrays include cDNA microarrays, oligo DNA microarrays, SNP microarrays, and BAC microarrays. 
  2. MMChips allow the analysis of cross-platform and between-laboratory data. It analyzes interactions between DNA and proteins. ChIP-chip and ChIP-seq are the two techniques used for this array. 
  3. Protein microarrays act as a platform for the identification of hundreds of thousands of proteins in a highly parallel manner. The three types of protein microarrays are functional protein microarrays, reverse-phase protein microarrays, and analytical protein microarrays. 
  4. Cellular microarrays, also called transfection microarrays, are used for screening large-scale chemical and genomic libraries and investigating the local cellular microenvironment. 
  5. Antibody microarrays, also called antibody chips, are protein-specific microarrays which contain a group of capture antibodies arranged inside a microscope slide. These arrays are used for detecting antigens. 
  6. Tissue microarrays are utilized to analyze the expression of proteins simultaneously in multiple tissue samples on one slide. They are made of small-tissue cores from areas of interest in paraffin-embedded tissues. 
  7. Peptide microarrays are used for the analysis or optimization of protein-protein interactions. It assists in antibody recognition by screening proteomes. 
  8. Phenotype microarrays are primarily used in drug development. They are able to quantitatively measure thousands of cellular phenotypes simultaneously. They are also used in functional genomics and toxicological testing.  

 

Additional resources

Microarrays

Gene Expression Analysis & Genotyping