What device is used to create thin sections of specimens for electron microscopy?
Posted March 16, 2023
Answer
An ultramicrotome is used to create thin sections of specimens for electron microscopy. This device consists of a sharp knife made of glass or diamond and a trough filled with distilled water. The knife is capable of cutting samples into ultra-thin slices of 100 nm thickness or even thinner. The cut sections are collected in the trough of water. From there they are moved to a copper grid to be studied under an electron microscope. The thin, semi-transparent cross-sections allow electrons to pass through the sample more easily. To make the samples easier to cut, they are first fixed, dehydrated, and embedded in hard resin.
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