Short-read sequencing is a type of next-generation sequencing (NGS). It involves breaking a genome into small fragments, usually 50 to 300 bases, before being sequenced. Short-read sequencing or SRS is a powerful tool for generating genomic data and is the most widely used form of NGS with a wide range of diagnostic applications. It is accurate, cost-effective and fast, capable of sequencing the entire genome in roughly one day.