CD96 is a type I transmembrane protein that belongs to the immunoglobulin superfamily. The mature protein is 64 kDa and has three extracellular Ig-like domains/loops and a relatively short 45 amino acid cytoplasmic domain. Although many functions of CD96 are unknown, it may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. Interaction with CD155 has also been shown to down-regulate CD96 on the surface of NK cells, potentially through receptor internalization. CD96, a biomarker for acute myeloid leukemia, is highly expressed in T-cell acute lymphoblastic leukemia and myelodysplastic syndromes.

Common detection and analytical methods include flow cytometry, SDS-PAGE, Western Blot mRNA analysis, multiplexed IHF, statistical analysis, and NK and T-cell activation studies.

Cell Types

• αβ T-cell
• γδ T-cells
• NK cells
• NKT cells

Reference

1. Dougall, W. C., Kurtulus, S., Smyth, M. J., & Anderson, A. C. (2017). TIGIT and CD96: new checkpoint receptor targets for cancer immunotherapy. Immunological reviews, 276(1), 112–120. https://doi.org/10.1111/imr.12518