What is produced when ATP is hydrolyzed?
Posted September 13, 2022
ATP becomes hydrolyzed to dissociate into ADP and Pi, and the free energy released during the process is lost as heat. After ATP hydrolysis, a phosphate from ATP is transferred onto another molecule (phosphorylation). The energy that becomes released by ATP and how it is used for work in the cell depends on energy coupling. Cells couple exergonic reactions of ATP hydrolysis with endergonic reactions. One example of energy coupling is the sodium potassium (Na+/K+) pump, as the pump expels sodium out of the cell and pushes potassium into the cell. The energy released from the hydrolysis of ATP is coupled with energy to fuel the NA+/K+ pump. It is important to note that ATP hydrolysis is a spontaneous process, as ATP is a very unstable molecule.
Molecular Mechanism of ATP Hydrolysis in an ABC Transporter
ATP assays for measuring cell proliferation
Biochemical assays for measuring ATP activity, formation or depletion