What is Sanger sequencing used for?

Sanger Sequencing is a technique used to identify the specific order and identity of nucleotides in a DNA molecule. Sanger Sequencing is also widely used in verifying known familial genetic variations, carrying out sequencing for specific individual genes, and confirming findings from NGS. For example, in cases where there is a known genetic variation within a family or a population, Sanger Sequencing is used to confirm the presence of these known familial variants. Families with a history of genetic disorders or specific mutations benefit from Sanger Sequencing to confirm whether individuals carry the expected genetic variations. Sanger Sequencing is also used for identifying point mutations or small deletion/duplications. Sanger Sequencing plays a crucial role in examining the range of mutations present in tumors. By designing primers for multiple regions (amplicons), researchers can make the sequencing method to cover specific areas of interest.