AAT Bioquest

What is the basic mechanism of enzyme reaction?

Posted May 19, 2023


An enzyme reaction is initiated when a substrate is attracted to the enzyme’s active site. Catalytic residues in the active site bind to the substrate via non-covalent bonds, eventually breaking down the substrate or combining the substrate with other molecules to form an enzyme-substrate complex. The enzyme allows the newly formed product to dissociate from its surface. It then returns to its original state and is free to bind to another molecule of the substrate. 

Additional resources

Do enzymes bind their substrates in the ground state because of a physico-chemical requirement?


Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*