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What is the main difference between preparing a sample for fluorescence microscopy versus light microscopy?
Posted March 16, 2023
Answer
The main difference is that a fluorochrome dye is used to prepare a sample for fluorescence microscopy, whereas preparing a sample for light microscopy involves fixation. Fixing samples helps to maintain the structure of the cell and the subcellular components for observation.
Additional resources
Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications