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What's the difference between SMCC and sulfo-SMCC?
Posted September 5, 2023
Crosslinkersamine-reactivemaleimide
Answer

Basis of differentiation

SMCC

Sulfo-SMCC

Functional groups

SMCC consists of two reactive functional groups: a maleimide group and a succinimidyl

Is an analogous compound to SMCC but with an added sulfo (sulfonate) group

Solubility

SMCC is typically used as a powder and needs to be dissolved in DMSO or DMF before use

Sulfo-SMCC is highly soluble in water and can be used in biological reactions without the need for organic solvents 

Applications

SMCC is used to crosslink thiol-containing molecules with amine-containing molecules

Sulfo-SMCC is suitable for aqueous based reactions 

Additional resources
Evaluation of the sulfo-succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate coupling chemistry for attachment of oligonucleotides to magnetic nanobeadsSMCC [4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester] *CAS 64987-85-5* Sulfo-SMCC [4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester, sodium salt] *CAS#: 92921-24-9*

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